7 edition of DNA Topoisomerase Protocols, Part II found in the catalog.
January 15, 2001
by Humana Press
Written in English
|Contributions||Neil Osheroff (Editor), Mary-Ann Bjornsti (Editor)|
|The Physical Object|
|Number of Pages||333|
The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through. In molecular biology Type I topoisomerases are enzymes that cut one of the two strands of double-stranded DNA, relax the strand, and reanneal the strand. They are further subdivided into two structurally and mechanistically distinct topoisomerases: type IA and type IB. Type IA topoisomerases change the linking number of a circular DNA strand by units of strictly 1.
Topoisomerase II (Top2) is an essential enzyme that decatenates DNA via a transient Top2-DNA cova-lent intermediate. This intermediate can be stabilized by a class of drugs termed Top2 poisons. The DNA topoisomerase I inhibitor irinotecan was approved initially in by Japanese authorities. Irinotecan is a prodrug that requires activation to become the metabolite, SN, which is inactivated by glucuronidation of the UGT1A1 isoform. In 30%–45% of patients, irinotecan is associated with severe side effects, such as neutropenia and.
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity). Involved in animal organ regeneration and chromosome segregation. Predicted to localize to DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex and nucleus. Is expressed in digestive system; head; nervous system; neural tube; and pectoral fin. Orthologous to human TOP2A (DNA topoisomerase II alpha). Genome Resources.
The Best Man (Harlequin Romance Larger Print)
Farm boys, artillery men
rape of the lock
Engineering solutions to industrial corrosion problems
Jains in the world
Like A Dog To Its Vomit
The 2007-2012 Outlook for Steel Heating Boilers with Maximum 15 P.s.i. and All Other Hot Water Heating Boilers Excluding Parts in the United States
My Sister Sophie
Russian naval terminology
Development of a Geographic Information System (GIS) Prototype Data Dictionary for Oil Spill Response Activities
Benjamin Beans family reunion
Developing effective education and awareness programmes
DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs (Methods in Molecular Biology) (v. 2): Medicine & Health Science Books @ DNA Topoisomerase Protocols, II: Enzymology and Drugs brings together an unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs.
DNA Topoisomerase Protocols, Part 1: DNA Topology and Enzymes (Methods in Molecular Biology, Vol. 94): Medicine & Health Science Books @ A companion volume, DNA Topoisomerase Protocols, v.
2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs.
14 rows Discovery. In the s, James C. Wang was the first to discover a topoisomerase when. Type II topoisomerases cut both strands of the DNA helix simultaneously in order to manage DNA tangles and use the hydrolysis of ATP, unlike Type I this process, these enzymes change the linking number of circular DNA by ±2.
DNA Topoisomerase Protocols Part II: Enzymology and Drugs METHODS IN MOLECULAR BIOLOGY TM John M. Walker, SERIES EDITOR Proteoglycan Protocols, edited by Renato V. Iozzo, DNA Arrays: Methods and Protocols, edited by Jang B. Rampal, Neurotrophin Protocols, edited by Robert A. Part II book Rush, Bjornsti, M.A.
and Osheroff, N. DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs Methods in Molecular Biology | Volume No.: 95, Print ISBN: Detailed protocols for many different aspects of DNA topoisomerases. Nitiss, J.L. DNA topoisomerase II and its growing repertoire of biological functions.
DNA topoisomerases represent an essential family of DNA processing enzymes and a large number of topoisomerase inhibitors are used clinically for the treatment of various human cancers. Novels drugs are in clinical development both against type I and type II topoisomerases.
The book will include. General description This is a collection of protocols for investigating DNA structure, topology, and DNA topoisomerase function. These methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid.
Buy DNA Topoisomerase Protocols: Enzymology and Drugs v. 2 (Methods in Molecular Biology) by Bjornsti, Mary Ann, Osheroff, Neil (ISBN: ) from Amazon's Book Store. Everyday low prices and free delivery on eligible : Mary Ann Bjornsti, Neil Osheroff.
Human Topoisomerase II Assay Kit Product Description. This kit is designed to allow quick and specific assays for eukaryotic type II DNA topoisomerases. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of type II topoisomerases.
Type II DNA topoisomerase enzymes (TOP2) catalyze topological changes by strand passage reactions. They involve passing one intact double stranded DNA duplex through a transient enzyme-bridged break in another (gated helix) followed by ligation of the break by TOP2.
A TOP2 poison, etoposide blocks TOP2 catalysis at the ligation step of the enzyme-bridged break, increasing the. DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Source: UniProtKB Ref.8 "Mutagenesis of E or K in the B' domain of human topoisomerase II beta increases the requirement for magnesium ions during strand passage.".
The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP.
Topoisomerase II has the tricky job of untangling the coiled DNA and nicking both strands of DNA using its two sets of 'jaws.' These strong jaws cleave, or split, not just one strand of DNA as in. Topotecan is a topoisomerase I inhibitor with a wide spectrum of activity.
Intraventricular administration of 1/th of the systemic dose of topotecan can lead to a fold greater CSF concentration.
In a phase II nonrandomized trial of 62 patients, intraventricular topotecan was given at mg twice weekly during 6 weeks (Groves et al. Heterochromatin is a transcriptionally repressive chromatin architecture that has a low abundance of genes but an enrichment of transposons. Defects in heterochromatin can cause the de-repression of genes and transposons, leading to deleterious physiological changes such as aging, cancer, and neurological disorders.
While the roles of topoisomerases in many DNA-based processes have been. An impressive array of expert authors highlight and review current advances in genome analysis to produce this invaluable, up-to-date and comprehensive overview of the methods currently employed for next-generation sequencing (NGS) data analysis.
The book highlights the problems and limitations, demonstrates the applications and indicates the developing trends in various fields of genome research. And finally, the last section includes several chapters describing the DNA repair pathways for topoisomerase-induced DNA damage.
This book is intended for students and faculty but also for health care professionals who wish to have a self-contained and up-to-date information on topoisomerases. Drugs that target DNA topoisomerase II (Top2), including etoposide (VP), doxorubicin, and mitoxantrone, are among the most effective anticancer drugs in clinical use.
However, Top2-based chemotherapy has been associated with higher incidences of secondary malignancies, notably the development of acute myeloid leukemia in VPtreated patients.DNA Topoisomerase Protocols, I: DNA Topology and Enzymes will be of immense value to the many basic scientists and clinicians who want better to understand and to exploit proficiently the wealth of recent discoveries about chromatin structure and its relation to gene expression, about DNA topoisomerases as the targets of antitumor and.
Topoisomerase type I cuts one strand whereas topoisomerase type II cuts both strands of the DNA to relax the coil and extend the DNA molecule. The regulation of DNA supercoiling is essential to DNA transcription and replication, when the DNA helix must unwind to permit the proper function of the enzymatic machinery involved in these processes.